MetaCyc Pathway: superpathway of sulfate assimilation and cysteine biosynthesis in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of sulfate assimilation and cysteine biosynthesis in Klebsiella michiganensis M5al

Phosphoglycerate dehydrogenase:
3-phospho-D-glycerate + NAD⁺→3-phosphooxypyruvate + NADH + H⁺ (EC 1.1.1.95)

Sulfate adenylyltransferase:
sulfate + ATP + H⁺→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)

Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H⁺ (EC 2.7.1.25)

Phosphoserine transaminase (in reverse):
3-phosphooxypyruvate + L-glutamate→O-phospho-L-serine + 2-oxoglutarate (EC 2.6.1.52)

Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H⁺ (EC 1.8.4.8)

Phosphoserine phosphatase:
O-phospho-L-serine + H₂O→L-serine + phosphate (EC 3.1.3.3)

Serine O-acetyltransferase:
L-serine + acetyl-CoA→O-acetyl-L-serine + coenzyme A (EC 2.3.1.30)

Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H⁺→hydrogen sulfide + 3 NADP⁺ + 3 H₂O (EC 1.8.1.2)

Cysteine synthase:
O-acetyl-L-serine + hydrogen sulfide→L-cysteine + acetate + H⁺ (EC 2.5.1.47)

Links:

Pathway details at MetaCyc
Fitness data for 23 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information