MetaCyc Pathway: (<i>S</i>)-lactate fermentation to propanoate, acetate and hydrogen in Klebsiella michiganensis M5al

MetaCyc Pathway: (S)-lactate fermentation to propanoate, acetate and hydrogen in Klebsiella michiganensis M5al

L-lactate dehydrogenase:
(S)-lactate + NAD⁺→pyruvate + NADH + H⁺ (EC 1.1.1.27)

Pyruvate carboxylase:
pyruvate + ATP + hydrogen carbonate→oxaloacetate + ADP + H⁺ + phosphate (EC 6.4.1.1)

No genes

Formate C-acetyltransferase (in reverse):
pyruvate + coenzyme A→acetyl-CoA + formate (EC 2.3.1.54)

formate + H⁺→H₂ + CO₂

Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H⁺→(S)-malate + NAD⁺ (EC 1.1.1.37; 1.1.1.38)

Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)

Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)

Fumarate hydratase:
(S)-malate→fumarate + H₂O (EC 4.2.1.2)

Succinate dehydrogenase (ubiquinone):
fumarate_[in] + a menaquinol→succinate_[in] + a menaquinone (EC 1.3.5.1)

succinate + propanoyl-CoA→succinyl-CoA + propanoate (EC 2.8.3.27)

Methylmalonyl-CoA mutase (in reverse):
succinyl-CoA→(R)-methylmalonyl-CoA (EC 5.4.99.2)

Methylmalonyl-CoA epimerase:
(R)-methylmalonyl-CoA→(S)-methylmalonyl-CoA (EC 5.1.99.1)

No genes

(S)-methylmalonyl-CoA_[in] + Na⁺_[in] + H⁺_[out]→propanoyl-CoA_[in] + CO₂_[in] + Na⁺_[out] (EC 7.2.4.3)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 39 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information