MetaCyc Pathway: superpathway of anaerobic sucrose degradation in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of anaerobic sucrose degradation in Klebsiella michiganensis M5al

Sucrose synthase (in reverse):
sucrose + H⁺ + UDP→β-D-fructofuranose + UDP-α-D-glucose (EC 2.4.1.13)

No genes

Fructokinase:
β-D-fructofuranose + ATP→β-D-fructofuranose 6-phosphate + ADP + H⁺ (EC 2.7.1.4)

UTP-monosaccharide-1-phosphate uridylyltransferase (in reverse):
UDP-α-D-glucose + diphosphate→α-D-glucopyranose 1-phosphate + H⁺ + UTP (EC 2.7.7.64; 2.7.7.9)

6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H⁺ (EC 2.7.1.11)

Phosphoglucomutase:
α-D-glucopyranose 1-phosphate→D-glucopyranose 6-phosphate (EC 5.4.2.2)

Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H⁺ (EC 1.2.1.12)

Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)

Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)

Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H₂O (EC 4.2.1.11)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

L-lactate dehydrogenase (in reverse):
pyruvate + NADH + H⁺→(S)-lactate + NAD⁺ (EC 1.1.1.27)

Pyruvate, water dikinase:
pyruvate + ATP + H₂O→phosphoenolpyruvate + AMP + 2 H⁺ + phosphate (EC 2.7.9.2)

Pyruvate decarboxylase:
pyruvate + H⁺→acetaldehyde + CO₂ (EC 4.1.1.1)

Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H⁺→ethanol + NAD⁺ (EC 1.1.1.1)

Links:

Pathway details at MetaCyc
Fitness data for 48 genes
Select organism:
All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information