MetaCyc Pathway: (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) in Klebsiella michiganensis M5al

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

BWI76_RS20460

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

BWI76_RS01365

BWI76_RS01370

BWI76_RS20455

Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H⁺→(3R)-3-hydroxybutanoyl-CoA + NADP⁺ (EC 1.1.1.36)

No genes

(S)-3-hydroxybutanoyl-CoA + H₂O→(S)-3-hydroxybutanoate + coenzyme A + H⁺

BWI76_RS06625

(3R)-3-hydroxybutanoyl-CoA + H₂O→(R)-3-hydroxybutanoate + coenzyme A + H⁺

BWI76_RS06625

Links:

Pathway details at MetaCyc
Fitness data for 10 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information