MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Klebsiella michiganensis M5al

Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)

Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)

Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H⁺→L-aspartate 4-semialdehyde + NADP⁺ + phosphate (EC 1.2.1.11)

Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H⁺→L-homoserine + NAD(P)⁺ (EC 1.1.1.3)

Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H⁺ (EC 2.7.1.39)

Threonine synthase:
O-phospho-L-homoserine + H₂O→L-threonine + phosphate (EC 4.2.3.1)

Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H₂O (EC 4.3.1.19)

1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

2-iminobutanoate + H₂O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

Acetolactate synthase:
2-oxobutanoate + H⁺ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ (EC 1.1.1.86)

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

Links:

Pathway details at MetaCyc
Fitness data for 37 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information