MetaCyc Pathway: superpathway of (<i>R,R</i>)-butanediol biosynthesis in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of (R,R)-butanediol biosynthesis in Klebsiella michiganensis M5al

Acetolactate synthase:
2 pyruvate + H⁺→(S)-2-acetolactate + CO₂ (EC 2.2.1.6)

Acetolactate decarboxylase:
(S)-2-acetolactate + H⁺→CO₂ + (R)-acetoin (EC 4.1.1.5)

(S)-2-acetolactate + an oxidized electron carrier + H⁺→CO₂ + diacetyl + a reduced two electron carrier (spontaneous)

No genes

diacetyl + NADH + H⁺→(R)-acetoin + NAD⁺ (EC 1.1.1.303)

(R,R)-butanediol dehydrogenase (in reverse):
(R)-acetoin + NADH + H⁺→(R,R)-2,3-butanediol + NAD⁺ (EC 1.1.1.4)

Links:

Pathway details at MetaCyc
Fitness data for 11 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information