MetaCyc Pathway: L-isoleucine degradation I in Klebsiella michiganensis M5al

MetaCyc Pathway: L-isoleucine degradation I in Klebsiella michiganensis M5al

Branched-chain-amino-acid transaminase:
L-isoleucine + 2-oxoglutarate→(3S)-3-methyl-2-oxopentanoate + L-glutamate (EC 2.6.1.42)

2-oxoisovalerate dehydrogenase (acylating):
(3S)-3-methyl-2-oxopentanoate + coenzyme A + NAD⁺→(2S)-2-methylbutanoyl-CoA + CO₂ + NADH (EC 1.2.1.25)

No genes

(2S)-2-methylbutanoyl-CoA + an oxidized electron-transfer flavoprotein + H⁺→(E)-2-methylcrotonoyl-CoA + a reduced electron-transfer flavoprotein (EC 1.3.8.5)

No genes

(E)-2-methylcrotonoyl-CoA + H₂O→(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (EC 4.2.1.150)

3-hydroxy-2-methylbutyryl-CoA dehydrogenase:
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD⁺→2-methylacetoacetyl-CoA + NADH + H⁺ (EC 1.1.1.178)

Acetyl-CoA C-acyltransferase (in reverse):
2-methylacetoacetyl-CoA + coenzyme A→acetyl-CoA + propanoyl-CoA (EC 2.3.1.16)

Links:

Pathway details at MetaCyc
Fitness data for 12 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information