MetaCyc Pathway: superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass in Klebsiella michiganensis M5al

MetaCyc Pathway: superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass in Klebsiella michiganensis M5al

D-glucopyranose_[out] + an [HPr protein]-N^π-phospho-L-histidine_[in]→D-glucopyranose 6-phosphate_[in] + an [HPr]-L-histidine_[in] (EC 2.7.1.199)

Glucose-6-phosphate isomerase:
α-D-glucose 6-phosphate→β-D-fructofuranose 6-phosphate (EC 5.3.1.9)

6-phosphofructokinase:
β-D-fructofuranose 6-phosphate + ATP→β-D-fructofuranose 1,6-bisphosphate + ADP + H⁺ (EC 2.7.1.11)

Fructose-bisphosphate aldolase:
β-D-fructofuranose 1,6-bisphosphate→glycerone phosphate + D-glyceraldehyde 3-phosphate (EC 4.1.2.13)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H⁺ (EC 1.2.1.12)

Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.11)

Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H₂O (EC 4.2.1.11)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

Pyruvate, water dikinase:
pyruvate + ATP + H₂O→phosphoenolpyruvate + AMP + 2 H⁺ + phosphate (EC 2.7.9.2)

pyruvate + coenzyme A + NAD⁺→acetyl-CoA + CO₂ + NADH (EC 1.2.1.104)

Citrate (Si)-synthase:
acetyl-CoA + oxaloacetate + H₂O→citrate + coenzyme A + H⁺ (EC 2.3.3.1; 2.3.3.16; 2.3.3.3)

Malate synthase:
acetyl-CoA + glyoxylate + H₂O→(S)-malate + coenzyme A + H⁺ (EC 2.3.3.9)

Aconitate hydratase:
citrate→cis-aconitate + H₂O (EC 4.2.1.3)

Aconitate hydratase:
cis-aconitate + H₂O→D-threo-isocitrate (EC 4.2.1.3)

Isocitrate lyase:
D-threo-isocitrate→glyoxylate + succinate (EC 4.1.3.1)

Isocitrate dehydrogenase (NADP(+)):
D-threo-isocitrate + NADP⁺→2-oxoglutarate + CO₂ + NADPH (EC 1.1.1.42)

2-oxoglutarate + coenzyme A + NAD⁺→succinyl-CoA + CO₂ + NADH (EC 1.2.1.105)

Succinate--CoA ligase (ADP-forming) (in reverse):
succinyl-CoA + ADP + phosphate→succinate + ATP + coenzyme A (EC 6.2.1.5)

Succinate dehydrogenase (ubiquinone):
succinate_[in] + an electron-transfer quinone→fumarate_[in] + an electron-transfer quinol (EC 1.3.5.1)

No genes

Fumarate hydratase (in reverse):
fumarate + H₂O→(S)-malate (EC 4.2.1.2)

Malate dehydrogenase:
(S)-malate + NAD⁺→oxaloacetate + NADH + H⁺ (EC 1.1.1.37; 1.1.1.38)

(S)-malate_[in] + an electron-transfer quinone→oxaloacetate_[in] + an electron-transfer quinol (EC 1.1.5.4)

Links:

Pathway details at MetaCyc
Fitness data for 56 genes
Select organism:
All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information