MetaCyc Pathway: gluconeogenesis I in Klebsiella michiganensis M5al

MetaCyc Pathway: gluconeogenesis I in Klebsiella michiganensis M5al

Malate dehydrogenase (oxaloacetate-decarboxylating):
(S)-malate + NAD⁺→pyruvate + CO₂ + NADH (EC 1.1.1.38)

Malate dehydrogenase:
(S)-malate + NAD⁺→oxaloacetate + NADH + H⁺ (EC 1.1.1.37; 1.1.1.38)

Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP⁺→pyruvate + CO₂ + NADPH (EC 1.1.1.40)

Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→phosphoenolpyruvate + ADP + CO₂ (EC 4.1.1.49)

Pyruvate, water dikinase:
pyruvate + ATP + H₂O→phosphoenolpyruvate + AMP + 2 H⁺ + phosphate (EC 2.7.9.2)

Phosphopyruvate hydratase (in reverse):
phosphoenolpyruvate + H₂O→2-phospho-D-glycerate (EC 4.2.1.11)

2-phospho-D-glycerate→3-phospho-D-glycerate (EC 5.4.2.12)

Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)

Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADH + H⁺→D-glyceraldehyde 3-phosphate + NAD⁺ + phosphate (EC 1.2.1.12)

Fructose-bisphosphate aldolase (in reverse):
D-glyceraldehyde 3-phosphate + glycerone phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)

Glucose-6-phosphate isomerase (in reverse):
β-D-fructofuranose 6-phosphate→α-D-glucose 6-phosphate (EC 5.3.1.9)

Links:

Pathway details at MetaCyc
Fitness data for 28 genes
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All MetaCyc pathways for Klebsiella michiganensis M5al

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information