MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Herbaspirillum seropedicae SmR1

MetaCyc Pathway: 1-butanol autotrophic biosynthesis (engineered) in Herbaspirillum seropedicae SmR1

2 H₂O_[in] + hν_[cytosol] + 2 a plastoquinone + 4 H⁺_[out]→2 a plastoquinol + dioxygen_[in] + 4 H⁺_[in] (EC 1.10.3.9)

No genes

a plastoquinol + 2 an oxidized plastocyanin_[in] + 2 H⁺_[out]→2 a reduced plastocyanin_[in] + a plastoquinone + 4 H⁺_[in] (EC 7.1.1.6)

No genes

a reduced plastocyanin_[in] + hν_[cytosol] + an oxidized ferredoxin [iron-sulfur] cluster_[out]→a reduced ferredoxin [iron-sulfur] cluster_[out] + an oxidized plastocyanin_[in] (EC 1.97.1.12)

No genes

Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster_[out] + NADP⁺_[out] + H⁺_[out]→NADPH_[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster_[out] (EC 1.18.1.2)

Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H⁺→D-glyceraldehyde 3-phosphate + NADP⁺ + phosphate (EC 1.2.1.13)

No genes

Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate (EC 5.3.1.1)

Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate (EC 4.1.2.13)

Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate (EC 4.1.2.13)

Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H₂O→β-D-fructofuranose 6-phosphate + phosphate (EC 3.1.3.11)

Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H₂O→D-sedoheptulose 7-phosphate + phosphate (EC 3.1.3.37)

No genes

Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate (EC 2.2.1.1)

Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate (EC 2.2.1.1)

Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate (EC 5.3.1.6)

Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate (EC 5.1.3.1)

Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H⁺ (EC 2.7.1.19)

No genes

Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO₂ + H₂O→2 3-phospho-D-glycerate + 2 H⁺ (EC 4.1.1.39)

No genes

3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)

Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP (EC 2.7.2.3)

Phosphopyruvate hydratase:
2-phospho-D-glycerate→H₂O + phosphoenolpyruvate (EC 4.2.1.11)

Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H⁺→pyruvate + ATP (EC 2.7.1.40)

pyruvate + coenzyme A + NAD⁺→CO₂ + acetyl-CoA + NADH (EC 1.2.1.104)

Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)

3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H⁺→(S)-3-hydroxybutanoyl-CoA + NAD⁺ (EC 1.1.1.35)

(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H₂O (EC 4.2.1.150)

Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H⁺→butanoyl-CoA + NAD⁺ (EC 1.3.1.44)

Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H⁺→1-butanal + coenzyme A + NAD(P)⁺ (EC 1.2.1.57)

No genes

1-butanal + NADH + H⁺→butan-1-ol + NAD⁺

No genes

Links:

Pathway details at MetaCyc
Fitness data for 41 genes
Select organism:
All MetaCyc pathways for Herbaspirillum seropedicae SmR1

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information