MetaCyc Pathway: L-tryptophan biosynthesis in Dinoroseobacter shibae DFL-12

Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H⁺ + pyruvate (EC 4.1.3.27)

Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)

Dshi_1670

Dshi_1799

Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)

Dshi_1029

Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H⁺→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO₂ + H₂O (EC 4.1.1.48)

Dshi_1800

Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)

Dshi_0952

Dshi_1031

indole + L-serine→L-tryptophan + H₂O (EC 4.2.1.122; 4.2.1.20)

Dshi_0952

Dshi_1031

Links:

Pathway details at MetaCyc
Fitness data for 8 genes
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All MetaCyc pathways for Dinoroseobacter shibae DFL-12

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information