MetaCyc Pathway: L-isoleucine biosynthesis II in Dinoroseobacter shibae DFL-12

MetaCyc Pathway: L-isoleucine biosynthesis II in Dinoroseobacter shibae DFL-12

pyruvate + acetyl-CoA + H₂O→(R)-citramalate + coenzyme A + H⁺ (EC 2.3.3.21)

No genes

(R)-2-methylmalate dehydratase:
(R)-citramalate→citraconate + H₂O (EC 4.2.1.35)

citraconate + H₂O→(2R,3S)-3-methylmalate

(2R,3S)-3-methylmalate + NAD⁺→2-oxobutanoate + CO₂ + NADH

No genes

Acetolactate synthase:
2-oxobutanoate + pyruvate + H⁺→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)⁺ (EC 1.1.1.383)

No genes

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

Links:

Pathway details at MetaCyc
Fitness data for 12 genes
Select organism:
All MetaCyc pathways for Dinoroseobacter shibae DFL-12

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information