MetaCyc Pathway: protocatechuate degradation II (ortho-cleavage pathway) in Dinoroseobacter shibae DFL-12

Protocatechuate 3,4-dioxygenase: protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H+ (EC 1.13.11.3)

Dshi_3476

Dshi_3477

3-carboxy-cis,cis-muconate cycloisomerase (in reverse): 3-carboxy-cis,cis-muconate + H+→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2)

Dshi_3474

4-carboxymuconolactone decarboxylase: (2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H+→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO2 (EC 4.1.1.44)

Dshi_1323

Dshi_2361

Dshi_2969

Dshi_3478

3-oxoadipate enol-lactonase: (4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+ (EC 3.1.1.24)

Dshi_0866

Dshi_1007

Dshi_1435

Dshi_3138

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information