MetaCyc Pathway: L-ascorbate biosynthesis VIII (engineered pathway) in Ralstonia sp. UNC404CL21Col

MetaCyc Pathway: L-ascorbate biosynthesis VIII (engineered pathway) in Ralstonia sp. UNC404CL21Col

Quinoprotein glucose dehydrogenase:
D-glucopyranose_[out] + a ubiquinone_[pm-bac-neg]→D-glucono-1,5-lactone_[out] + a ubiquinol_[pm-bac-neg] (EC 1.1.5.2)

No genes

Gluconolactonase:
D-glucono-1,5-lactone + H₂O→D-gluconate + H⁺ (spontaneous) (EC 3.1.1.17)

Gluconate 2-dehydrogenase (acceptor):
D-gluconate + an oxidized electron carrier→2-keto-D-gluconate + a reduced two electron carrier (EC 1.1.99.3)

2,5-didehydrogluconate reductase:
2-keto-D-gluconate + NADP⁺→2,5-didehydro-D-gluconate + NADPH + H⁺ (EC 1.1.1.274)

Dehydrogluconate dehydrogenase:
2-keto-D-gluconate + an oxidized electron carrier→2,5-didehydro-D-gluconate + a reduced two electron carrier (EC 1.1.99.4)

2,5-didehydro-D-gluconate + NADPH + H⁺→2-keto-L-gulonate + NADP⁺ (EC 1.1.1.346)

2-keto-L-gulonate→L-ascorbate + H₂O

No genes

Links:

Pathway details at MetaCyc
Fitness data for 10 genes
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All MetaCyc pathways for Ralstonia sp. UNC404CL21Col

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information