MetaCyc Pathway: methyl ketone biosynthesis (engineered) in Ralstonia sp. UNC404CL21Col

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a fatty acid + ATP + coenzyme A→an acyl-CoA + AMP + diphosphate
No genes
Acyl-CoA oxidase:
a 2,3,4-saturated fatty acyl CoA + dioxygen→a (2E)-2-enoyl-CoA + hydrogen peroxide
(EC 1.3.3.6)
No genes
Enoyl-CoA hydratase (in reverse):
a (2E)-2-enoyl-CoA + H2O→a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA
(EC 4.2.1.17)
ABZR87_RS02095
ABZR87_RS02100
ABZR87_RS06190
ABZR87_RS06470
ABZR87_RS07480
ABZR87_RS07495
ABZR87_RS11295
ABZR87_RS12585
ABZR87_RS14655
ABZR87_RS14660
ABZR87_RS16545
ABZR87_RS16935
ABZR87_RS17480
ABZR87_RS17485
ABZR87_RS20520
ABZR87_RS20900
ABZR87_RS21810
ABZR87_RS23090
ABZR87_RS23345
3-hydroxyacyl-CoA dehydrogenase:
a 2,3,4-saturated (3S)-3-hydroxyacyl-CoA + NAD+→a 2,3,4-saturated 3-oxoacyl-CoA + NADH + H+
(EC 1.1.1.35)
ABZR87_RS00745
ABZR87_RS07480
ABZR87_RS12585
ABZR87_RS14655
ABZR87_RS23345
a 2,3,4-saturated 3-oxoacyl-CoA + H2O→a 3-oxoacid + coenzyme A + H+
No genes
a 3-oxoacid + H+→a methyl ketone + CO2 (spontaneous)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information