MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Paraburkholderia bryophila 376MFSha3.1

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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
H281DRAFT_00306
H281DRAFT_00356
H281DRAFT_02406
H281DRAFT_03007
H281DRAFT_03642
H281DRAFT_04551
H281DRAFT_04552
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
H281DRAFT_04553
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
H281DRAFT_00127
H281DRAFT_02329
H281DRAFT_03422
H281DRAFT_05312
H281DRAFT_05919
H281DRAFT_06137
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
H281DRAFT_01050
H281DRAFT_01171
H281DRAFT_01251
H281DRAFT_02579
H281DRAFT_02988
H281DRAFT_03803
H281DRAFT_04166
H281DRAFT_05379
H281DRAFT_05508

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information