MetaCyc Pathway: mandelate degradation to acetyl-CoA in Paraburkholderia bryophila 376MFSha3.1

Add experiment(s):


Mandelate racemase (in reverse):
(R)-mandelate→(S)-mandelate
(EC 5.1.2.2)
H281DRAFT_05472
(S)-mandelate dehydrogenase:
(S)-mandelate + an oxidized electron carrier→phenylglyoxylate + a reduced two electron carrier
(EC 1.1.99.31)
No genes
Benzoylformate decarboxylase:
phenylglyoxylate + H+→benzaldehyde + CO2
(EC 4.1.1.7)
No genes
Benzaldehyde dehydrogenase (NAD(+)):
benzaldehyde + NAD+ + H2O→benzoate + NADH + 2 H+
(EC 1.2.1.28)
No genes
Benzaldehyde dehydrogenase (NADP(+)):
benzaldehyde + NADP+ + H2O→benzoate + NADPH + 2 H+
(EC 1.2.1.7)
No genes
Benzoate 1,2-dioxygenase:
benzoate + NADH + dioxygen + H+→3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+
(EC 1.14.12.10)
H281DRAFT_01075
H281DRAFT_01076
H281DRAFT_01077
H281DRAFT_01653
H281DRAFT_01654
H281DRAFT_02475
1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase:
3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+→catechol + CO2 + NADH
(EC 1.3.1.25)
H281DRAFT_01078
Catechol 1,2-dioxygenase:
catechol + dioxygencis,cis-muconate + 2 H+
(EC 1.13.11.1)
H281DRAFT_01051
H281DRAFT_01074
H281DRAFT_01643
H281DRAFT_02578
Catechol 2,3-dioxygenase:
catechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
(EC 1.13.11.2)
No genes
2-hydroxymuconate-semialdehyde hydrolase:
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + formate + H+
(EC 3.7.1.9)
H281DRAFT_05418
H281DRAFT_05456
Muconate cycloisomerase (in reverse):
cis,cis-muconate + H+→(+)-muconolactone
(EC 5.5.1.1)
H281DRAFT_01072
H281DRAFT_01645
H281DRAFT_06497
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate
(EC 4.2.1.80)
H281DRAFT_01663
H281DRAFT_03564
H281DRAFT_05231
Muconolactone Delta-isomerase:
(+)-muconolactone→(4,5-dihydro-5-oxofuran-2-yl)-acetate
(EC 5.3.3.4)
H281DRAFT_01071
H281DRAFT_01644
3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+
(EC 3.1.1.24)
H281DRAFT_01594
H281DRAFT_02073
H281DRAFT_02672
H281DRAFT_03639
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate
(EC 4.1.3.39)
H281DRAFT_01666
3-oxoadipate CoA-transferase:
3-oxoadipate + succinyl-CoA→3-oxoadipoyl-CoA + succinate
(EC 2.8.3.6)
H281DRAFT_01596
H281DRAFT_01597
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+
(EC 1.2.1.10)
H281DRAFT_01665
3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→acetyl-CoA + succinyl-CoA
(EC 2.3.1.174)
H281DRAFT_01504
H281DRAFT_05723

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information