MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Paraburkholderia bryophila 376MFSha3.1

Add experiment(s):


Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
H281DRAFT_00273
H281DRAFT_00432
H281DRAFT_00809
H281DRAFT_00920
H281DRAFT_01048
H281DRAFT_01557
H281DRAFT_01577
H281DRAFT_02293
H281DRAFT_02907
H281DRAFT_03132
H281DRAFT_03686
H281DRAFT_03705
H281DRAFT_04024
H281DRAFT_04101
H281DRAFT_04597
H281DRAFT_05051
H281DRAFT_05099
H281DRAFT_05339
H281DRAFT_05564
H281DRAFT_05880
H281DRAFT_06094
H281DRAFT_06439
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
H281DRAFT_00550
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
H281DRAFT_06071
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
H281DRAFT_00810
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
H281DRAFT_06029
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
H281DRAFT_00811
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
H281DRAFT_00878
H281DRAFT_01167
H281DRAFT_01887
H281DRAFT_04028
H281DRAFT_04606
H281DRAFT_05256
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
H281DRAFT_00878
H281DRAFT_01167
H281DRAFT_01887
H281DRAFT_02886
H281DRAFT_04028
H281DRAFT_04606
H281DRAFT_05256
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
H281DRAFT_00878
H281DRAFT_01167
H281DRAFT_01887
H281DRAFT_02886
H281DRAFT_04028
H281DRAFT_04606
H281DRAFT_05256
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
H281DRAFT_00306
H281DRAFT_00356
H281DRAFT_02406
H281DRAFT_03007
H281DRAFT_03642
H281DRAFT_04551
H281DRAFT_04552
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
H281DRAFT_04553
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
H281DRAFT_00127
H281DRAFT_02329
H281DRAFT_03422
H281DRAFT_05312
H281DRAFT_05919
H281DRAFT_06137
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
H281DRAFT_04193
H281DRAFT_05498

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information