MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Paraburkholderia bryophila 376MFSha3.1

MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Paraburkholderia bryophila 376MFSha3.1

Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H₂O (EC 4.3.1.19)

H281DRAFT_00878

H281DRAFT_01167

H281DRAFT_01887

H281DRAFT_04028

H281DRAFT_04606

H281DRAFT_05256

1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

H281DRAFT_00878

H281DRAFT_01167

H281DRAFT_01887

H281DRAFT_02886

H281DRAFT_04028

H281DRAFT_04606

H281DRAFT_05256

2-iminobutanoate + H₂O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)

H281DRAFT_00878

H281DRAFT_01167

H281DRAFT_01887

H281DRAFT_02886

H281DRAFT_04028

H281DRAFT_04606

H281DRAFT_05256

Acetolactate synthase:
2-oxobutanoate + H⁺ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO₂ (EC 2.2.1.6)

H281DRAFT_00306

H281DRAFT_00356

H281DRAFT_02406

H281DRAFT_03007

H281DRAFT_03642

H281DRAFT_04551

H281DRAFT_04552

Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H⁺→(R)-2,3-dihydroxy-3-methylpentanoate + NADP⁺ (EC 1.1.1.86)

H281DRAFT_04553

Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H₂O (EC 4.2.1.9)

H281DRAFT_00127

H281DRAFT_02329

H281DRAFT_03422

H281DRAFT_05312

H281DRAFT_05919

H281DRAFT_06137

Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)

H281DRAFT_04193

H281DRAFT_05498

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information