MetaCyc Pathway: even <i>iso</i>-branched-chain fatty acid biosynthesis in Burkholderia phytofirmans PsJN

MetaCyc Pathway: even iso-branched-chain fatty acid biosynthesis in Burkholderia phytofirmans PsJN

Branched-chain-amino-acid transaminase:
L-valine + 2-oxoglutarate→3-methyl-2-oxobutanoate + L-glutamate (EC 2.6.1.42)

2-oxoisovalerate dehydrogenase (acylating):
3-methyl-2-oxobutanoate + coenzyme A + NAD⁺→isobutanoyl-CoA + CO₂ + NADH (EC 1.2.1.25)

No genes

isobutanoyl-CoA + a malonyl-[acp] + H⁺→a 4-methyl-3-oxopentanoyl-[acp] + CO₂ + coenzyme A (EC 2.3.1.300)

No genes

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 4-methyl-3-oxopentanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-4-methylpentanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-4-methylpentanoyl-[acp]→a (2E)-4-methylpent-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADH + H⁺→a 5-methylhexanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-4-methylpent-2-enoyl-[acp] + NADPH + H⁺→a 5-methylhexanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104; 1.3.1.39)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 5-methylhexanoyl-[acp] + a malonyl-[acp] + H⁺→a 6-methyl-3-oxoheptanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 6-methyl-3-oxoheptanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-6-methylheptanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-6-methylheptanoyl-[acp]→a (2E)-6-methylhept-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADH + H⁺→a 6-methylheptanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-6-methylhept-2-enoyl-[acp] + NADPH + H⁺→a 6-methylheptanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 6-methylheptanoyl-[acp] + a malonyl-[acp] + H⁺→an 8-methyl-3-oxononanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
an 8-methyl-3-oxononanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-8-methylnonanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-8-methylnonanoyl-[acp]→a (2E)-8-methylnon-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADH + H⁺→an 8-methylnonanoyl-[acyl-carrier protein] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-8-methylnon-2-enoyl-[acp] + NADPH + H⁺→an 8-methylnonanoyl-[acyl-carrier protein] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
an 8-methylnonanoyl-[acyl-carrier protein] + a malonyl-[acp] + H⁺→a 1o-methyl-3-oxoundecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 1o-methyl-3-oxoundecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-10-methylundecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-10-methylundecanoyl-[acp]→a (2E)-10-methylundec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADH + H⁺→an 10-methylundecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-10-methylundec-2-enoyl-[acp] + NADPH + H⁺→an 10-methylundecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
an 10-methylundecanoyl-[acp] + a malonyl-[acp] + H⁺→a 12-methyl-3-oxotridecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 12-methyl-3-oxotridecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-12-methyltridecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-12-methyltridecanoyl-[acp]→a (2E)-12-methyltridec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADH + H⁺→a 12-methyltridecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-12-methyltridec-2-enoyl-[acp] + NADPH + H⁺→a 12-methyltridecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.104)

Beta-ketoacyl-acyl-carrier-protein synthase II:
a 12-methyltridecanoyl-[acp] + a malonyl-[acp] + H⁺→a 14-methyl-3-oxopentadecanoyl-[acp] + a soluble [acyl-carrier protein] + CO₂ (EC 2.3.1.179)

Oleoyl-[acyl-carrier-protein] hydrolase:
a 12-methyltridecanoyl-[acp] + H₂O→12-methyltridecanoate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

No genes

3-oxoacyl-[acyl-carrier-protein] reductase (in reverse):
a 14-methyl-3-oxopentadecanoyl-[acp] + NADPH + H⁺→a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp] + NADP⁺ (EC 1.1.1.100)

3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase:
a (3R)-3-hydroxy-14-methylpentadecanoyl-[acp]→a (2E)-14-methylpentadec-2-enoyl-[acp] + H₂O (EC 4.2.1.59)

Enoyl-[acyl-carrier-protein] reductase (NADH) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADH + H⁺→a 14-methylpentadecanoyl-[acp] + NAD⁺ (EC 1.3.1.9)

Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) (in reverse):
a (2E)-14-methylpentadec-2-enoyl-[acp] + NADPH + H⁺→a 14-methylpentadecanoyl-[acp] + NADP⁺ (EC 1.3.1.10; 1.3.1.39)

Oleoyl-[acyl-carrier-protein] hydrolase:
a 14-methylpentadecanoyl-[acp] + H₂O→14-methylpentadecanoate + a soluble [acyl-carrier protein] + H⁺ (EC 3.1.2.14)

No genes

Links:

Pathway details at MetaCyc
Fitness data for 54 genes
Select organism:
All MetaCyc pathways for Burkholderia phytofirmans PsJN

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information