MetaCyc Pathway: butanol and isobutanol biosynthesis (engineered) in Burkholderia phytofirmans PsJN

Add experiment(s):


Glycine oxidase:
glycine + dioxygen + H2O→glyoxylate + ammonium + hydrogen peroxide (EC 1.4.3.19; 1.4.3.3)
BPHYT_RS01320
BPHYT_RS17785
BPHYT_RS22645
3-ethylmalate synthase:
glyoxylate + butanoyl-CoA + H2O→3-ethylmalate + coenzyme A + H+ (EC 2.3.3.7)
BPHYT_RS12305
3-isopropylmalate dehydrogenase:
3-ethylmalate + NAD+→2-oxovalerate + CO2 + NADH (EC 1.1.1.85)
BPHYT_RS33900
2-oxovalerate→3-methyl-2-oxobutanoate
No genes
Pyruvate decarboxylase:
2-oxovalerate + H+→1-butanal + CO2 (EC 4.1.1.1)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
BPHYT_RS00510
BPHYT_RS16150
BPHYT_RS20205
BPHYT_RS24470
BPHYT_RS25430
BPHYT_RS27390
BPHYT_RS28135
BPHYT_RS30075
BPHYT_RS31305
BPHYT_RS31860

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information