MetaCyc Pathway: L-ascorbate biosynthesis VIII (engineered pathway) in Burkholderia phytofirmans PsJN

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Quinoprotein glucose dehydrogenase:
D-glucopyranose[out] + a ubiquinone[pm-bac-neg]→D-glucono-1,5-lactone[out] + a ubiquinol[pm-bac-neg] (EC 1.1.5.2)
BPHYT_RS08325
Gluconolactonase:
D-glucono-1,5-lactone + H2O→D-gluconate + H+ (spontaneous) (EC 3.1.1.17)
BPHYT_RS21245
BPHYT_RS24170
Gluconate 2-dehydrogenase (acceptor):
D-gluconate + an oxidized electron carrier→2-keto-D-gluconate + a reduced two electron carrier (EC 1.1.99.3)
BPHYT_RS01065
BPHYT_RS01070
BPHYT_RS23020
BPHYT_RS23025
BPHYT_RS23030
BPHYT_RS30775
BPHYT_RS30780
BPHYT_RS30785
2,5-didehydrogluconate reductase:
2-keto-D-gluconate + NADP+→2,5-didehydro-D-gluconate + NADPH + H+ (EC 1.1.1.274)
No genes
Dehydrogluconate dehydrogenase:
2-keto-D-gluconate + an oxidized electron carrier→2,5-didehydro-D-gluconate + a reduced two electron carrier (EC 1.1.99.4)
BPHYT_RS33755
2,5-didehydro-D-gluconate + NADPH + H+→2-keto-L-gulonate + NADP+ (EC 1.1.1.346)
No genes
2-keto-L-gulonate→L-ascorbate + H2O
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information