MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Burkholderia phytofirmans PsJN

Add experiment(s):


2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
BPHYT_RS02790
BPHYT_RS06570
BPHYT_RS13280
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
BPHYT_RS13515
BPHYT_RS13520
BPHYT_RS23060
BPHYT_RS33905
BPHYT_RS33915
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
BPHYT_RS13515
BPHYT_RS13520
BPHYT_RS23060
BPHYT_RS33905
BPHYT_RS33915
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
BPHYT_RS33900
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
BPHYT_RS33900
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
BPHYT_RS00510
BPHYT_RS16150
BPHYT_RS20205
BPHYT_RS24470
BPHYT_RS25430
BPHYT_RS27390
BPHYT_RS28135
BPHYT_RS30075
BPHYT_RS31305
BPHYT_RS31860

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information