MetaCyc Pathway: toluene degradation V (aerobic) (via toluene-cis-diol) in Burkholderia phytofirmans PsJN

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Toluene dioxygenase:
toluene + NADH + dioxygen + H+→(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
(EC 1.14.12.11)
No genes
Cis-1,2-dihydrobenzene-1,2-diol dehydrogenase:
(1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+→3-methylcatechol + NADH + H+
(EC 1.3.1.19)
No genes
Catechol 2,3-dioxygenase:
3-methylcatechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H+
(EC 1.13.11.2)
No genes
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + acetate + H+ (EC 3.7.1.25)
No genes
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate
(EC 4.2.1.80)
BPHYT_RS07240
BPHYT_RS11195
BPHYT_RS28745
BPHYT_RS34430
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate
(EC 4.1.3.39)
BPHYT_RS07250
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+
(EC 1.2.1.10)
BPHYT_RS07245

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information