MetaCyc Pathway: L-isoleucine biosynthesis IV in Burkholderia phytofirmans PsJN

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Propionate--CoA ligase:
propanoate + ATP + coenzyme A→propanoyl-CoA + AMP + diphosphate (EC 6.2.1.17)
BPHYT_RS07000
BPHYT_RS13830
BPHYT_RS27780
3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) (in reverse):
propanoyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxobutanoate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.7)
No genes
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
BPHYT_RS06145
BPHYT_RS06545
BPHYT_RS06550
BPHYT_RS10500
BPHYT_RS19345
BPHYT_RS31395
BPHYT_RS34970
(S)-2-aceto-2-hydroxybutanoate + NAD(P)H + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NAD(P)+ (EC 1.1.1.383)
No genes
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
BPHYT_RS04815
BPHYT_RS10100
BPHYT_RS19730
BPHYT_RS21270
BPHYT_RS25650
BPHYT_RS31695
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
BPHYT_RS16285

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information