MetaCyc Pathway: L-glutamate degradation V (via hydroxyglutarate) in Burkholderia phytofirmans PsJN

Add experiment(s):


Glutamate dehydrogenase:
L-glutamate + NAD+ + H2O→2-oxoglutarate + ammonium + NADH + H+ (EC 1.4.1.2)
BPHYT_RS16705
BPHYT_RS22475
2-oxoglutarate + NADH + H+→(R)-2-hydroxyglutarate + NAD+ (EC 1.1.1.399)
No genes
Glutaconate CoA-transferase:
(R)-2-hydroxyglutarate + acetyl-CoA→(R)-2-hydroxyglutaryl-CoA + acetate (EC 2.8.3.12)
No genes
(R)-2-hydroxyglutaryl-CoA→(E)-glutaconyl-CoA + H2O (EC 4.2.1.167)
No genes
(E)-glutaconyl-CoA[in] + Na+[in] + H+[in]→crotonyl-CoA[in] + CO2[in] + Na+[out] (EC 7.2.4.5)
No genes
crotonyl-CoA + H2O→(S)-3-hydroxybutanoyl-CoA (EC 4.2.1.150)
BPHYT_RS02345
BPHYT_RS03225
BPHYT_RS03235
BPHYT_RS03825
BPHYT_RS08375
BPHYT_RS08630
BPHYT_RS09560
BPHYT_RS10815
BPHYT_RS11780
BPHYT_RS13545
BPHYT_RS14830
BPHYT_RS25360
BPHYT_RS25695
BPHYT_RS27105
BPHYT_RS27895
BPHYT_RS28620
BPHYT_RS35465
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Propionate CoA-transferase:
butanoyl-CoA + acetate→acetyl-CoA + butanoate (EC 2.8.3.1; 2.8.3.8)
BPHYT_RS07875
BPHYT_RS20785
3-hydroxyacyl-CoA dehydrogenase:
(S)-3-hydroxybutanoyl-CoA + NAD+→acetoacetyl-CoA + NADH + H+ (EC 1.1.1.35)
BPHYT_RS03225
BPHYT_RS06760
BPHYT_RS09560
BPHYT_RS13545
BPHYT_RS28020
BPHYT_RS34155
Acetyl-CoA C-acyltransferase (in reverse):
acetoacetyl-CoA + coenzyme A→2 acetyl-CoA (EC 2.3.1.16; 2.3.1.9)
BPHYT_RS02230
BPHYT_RS03230
BPHYT_RS08275
BPHYT_RS09150
BPHYT_RS09180
BPHYT_RS09565
BPHYT_RS17345
BPHYT_RS21955
BPHYT_RS29385

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information