MetaCyc Pathway: L-histidine biosynthesis in Burkholderia phytofirmans PsJN

MetaCyc Pathway: L-histidine biosynthesis in Burkholderia phytofirmans PsJN

ATP phosphoribosyltransferase (in reverse):
5-phospho-α-D-ribose 1-diphosphate + ATP→1-(5-phospho-β-D-ribosyl)-ATP + diphosphate (EC 2.4.2.17)

Phosphoribosyl-ATP diphosphatase:
1-(5-phospho-β-D-ribosyl)-ATP + H₂O→1-(5-phospho-β-D-ribosyl)-AMP + diphosphate + H⁺ (EC 3.6.1.31)

Phosphoribosyl-AMP cyclohydrolase:
1-(5-phospho-β-D-ribosyl)-AMP + H₂O→1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide (EC 3.5.4.19)

isomerase:
1-(5-phospho-β-D-ribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide→phosphoribulosylformimino-AICAR-phosphate (EC 5.3.1.16)

phosphoribulosylformimino-AICAR-phosphate + L-glutamine→5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate + L-glutamate + H⁺ (EC 4.3.2.10)

Imidazoleglycerol-phosphate dehydratase:
D-erythro-1-(imidazol-4-yl)-glycerol 3-phosphate→3-(imidazol-4-yl)-2-oxopropyl phosphate + H₂O (EC 4.2.1.19)

Histidinol-phosphate transaminase (in reverse):
3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate→L-histidinol phosphate + 2-oxoglutarate (EC 2.6.1.9)

Histidinol-phosphatase:
L-histidinol phosphate + H₂O→histidinol + phosphate (EC 3.1.3.15)

Histidinol dehydrogenase:
histidinol + NAD⁺→histidinal + NADH + H⁺ (EC 1.1.1.23)

Histidinol dehydrogenase:
histidinal + NAD⁺ + H₂O→L-histidine + NADH + 2 H⁺ (EC 1.1.1.23)

Links:

Pathway details at MetaCyc
Fitness data for 15 genes
Select organism:
All MetaCyc pathways for Burkholderia phytofirmans PsJN

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information