MetaCyc Pathway: glycerol degradation to butanol in Shewanella sp. ANA-3

Add experiment(s):


Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
No genes
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
No genes
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
Shewana3_1026
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
Shewana3_0534
Shewana3_2122
Shewana3_2124
Shewana3_2330
Shewana3_3352
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
Shewana3_3351 (pgk)
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
Shewana3_0051
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
Shewana3_1116 (eno)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
Shewana3_2153
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
No genes
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
Shewana3_0023 (fadA)
Shewana3_1460 (fadI)
Shewana3_2771
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
Shewana3_0024 (fadB)
Shewana3_1461 (fadJ)
Shewana3_2768
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
Shewana3_0024 (fadB)
Shewana3_0572
Shewana3_0721
Shewana3_1461 (fadJ)
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
Shewana3_0261

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information