MetaCyc Pathway: superpathway of heme <i>b</i> biosynthesis from glutamate in Shewanella sp. ANA-3

MetaCyc Pathway: superpathway of heme b biosynthesis from glutamate in Shewanella sp. ANA-3

Glutamate--tRNA ligase:
L-glutamate + ATP + a tRNA^glu→an L-glutamyl-[tRNA^Glu] + AMP + diphosphate (EC 6.1.1.17)

Shewana3_1418 (gltX)

Glutamyl-tRNA reductase (in reverse):
an L-glutamyl-[tRNA^Glu] + NADPH + H⁺→(S)-4-amino-5-oxopentanoate + a tRNA^glu + NADP⁺ (EC 1.2.1.70)

Shewana3_0768 (hemA)

Glutamate-1-semialdehyde 2,1-aminomutase:
(S)-4-amino-5-oxopentanoate→5-aminolevulinate (EC 5.4.3.8)

Porphobilinogen synthase:
2 5-aminolevulinate→porphobilinogen + H⁺ + 2 H₂O (EC 4.2.1.24)

Hydroxymethylbilane synthase:
4 porphobilinogen + H₂O→preuroporphyrinogen + 4 ammonium (EC 2.5.1.61)

Shewana3_0386 (hemC)

Uroporphyrinogen-III synthase:
preuroporphyrinogen→uroporphyrinogen-III + H₂O (EC 4.2.1.75)

Uroporphyrinogen decarboxylase:
uroporphyrinogen-III + 4 H⁺→coproporphyrinogen III + 4 CO₂ (EC 4.1.1.37)

Shewana3_0435 (hemE)

Coproporphyrinogen oxidase:
coproporphyrinogen III + dioxygen + 2 H⁺→protoporphyrinogen IX + 2 CO₂ + 2 H₂O (EC 1.3.3.3)

Protoporphyrinogen oxidase:
protoporphyrinogen IX + 3 dioxygen→protoporphyrin IX + 3 hydrogen peroxide (EC 1.3.3.4)

protoporphyrin IX + Fe²⁺→protoheme + 2 H⁺ (EC 4.98.1.1)

Shewana3_2440 (hemH)

Links:

Pathway details at MetaCyc
Fitness data for 15 genes
Select organism:
All MetaCyc pathways for Shewanella sp. ANA-3

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information