MetaCyc Pathway: L-arginine biosynthesis II (acetyl cycle) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Uridine (N)
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Glutamate--ammonia ligase:
L-glutamate + ammonium + ATP→L-glutamine + ADP + H+ + phosphate (EC 6.3.1.2)
Pf6N2E2_2177 +0.8
Pf6N2E2_2730 +0.0
Pf6N2E2_3821 N.D.
Pf6N2E2_4509 -4.3
Pf6N2E2_4511 -0.5
Pf6N2E2_5686 -0.0
Amino-acid N-acetyltransferase:
L-glutamate + acetyl-CoA→N-acetyl-L-glutamate + coenzyme A + H+ (EC 2.3.1.1)
Pf6N2E2_2242 N.D.
Pf6N2E2_4286 +0.1
Pf6N2E2_4497 N.D.
Pf6N2E2_5516 N.D.
Acetylglutamate kinase:
N-acetyl-L-glutamate + ATP→N-acetylglutamyl-phosphate + ADP (EC 2.7.2.8)
Pf6N2E2_4373 N.D.
Carbamoyl-phosphate synthase (glutamine-hydrolyzing):
L-glutamine + 2 ATP + hydrogen carbonate + H2O→L-glutamate + carbamoyl phosphate + 2 ADP + 2 H+ + phosphate (EC 6.3.5.5)
Pf6N2E2_3364 N.D.
Pf6N2E2_3365 N.D.
N-acetyl-gamma-glutamyl-phosphate reductase (in reverse):
N-acetylglutamyl-phosphate + NADPH + H+→N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate (EC 1.2.1.38)
Pf6N2E2_1484 +0.4
Pf6N2E2_4836 -1.0
Ornithine carbamoyltransferase:
carbamoyl phosphate + L-ornithine→L-citrulline + H+ + phosphate (EC 2.1.3.3)
Pf6N2E2_2909 -0.2
Pf6N2E2_5579 -2.7
Acetylornithine transaminase (in reverse):
N-acetyl-L-glutamate 5-semialdehyde + L-glutamate→N-acetyl-L-ornithine + 2-oxoglutarate (EC 2.6.1.11)
Pf6N2E2_373 -0.3
Pf6N2E2_5326 +0.5
Pf6N2E2_5665 -0.5
Argininosuccinate synthase:
L-citrulline + ATP + L-aspartate→L-arginino-succinate + AMP + diphosphate + H+ (EC 6.3.4.5)
Pf6N2E2_5590 N.D.
Argininosuccinate lyase:
L-arginino-succinate→L-arginine + fumarate (EC 4.3.2.1)
Pf6N2E2_1798 +0.2
Pf6N2E2_4429 N.D.
Glutamate N-acetyltransferase:
L-glutamate + N-acetyl-L-ornithine→N-acetyl-L-glutamate + L-ornithine (EC 2.3.1.35)
Pf6N2E2_5516 N.D.

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information