MetaCyc Pathway: C4 photosynthetic carbon assimilation cycle, PEPCK type in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cadmium chloride hemipentahydrate 0.000015625 M
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Carbonate dehydratase (in reverse):
CO2 + H2O→hydrogen carbonate + H+ (EC 4.2.1.1)
Pf6N2E2_402 +0.3
Pf6N2E2_4095 +1.1
Pf6N2E2_5190 -0.1
Pf6N2E2_920 -0.1
Phosphoenolpyruvate carboxylase (in reverse):
hydrogen carbonate + phosphoenolpyruvate→oxaloacetate + phosphate (EC 4.1.1.31)
Pf6N2E2_3103 +0.0
Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf6N2E2_1496 -0.1
Pf6N2E2_2127 +2.4
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 -0.3
Pf6N2E2_3173 +0.2
Pf6N2E2_4288 +0.1
Pf6N2E2_4386 -0.1
Pf6N2E2_462 +0.0
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 +0.0
Pf6N2E2_5890 -0.1
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 -0.0
Pf6N2E2_684 -0.0
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) (in reverse):
oxaloacetate + NADPH + H+→(S)-malate + NADP+ (EC 1.1.1.40; 1.1.1.82)
Pf6N2E2_3762 +0.4
L-aspartate[in]→L-aspartate[out]
No genes
(S)-malate[in]→(S)-malate[out]
No genes
Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)):
(S)-malate + NADP+→CO2 + pyruvate + NADPH (EC 1.1.1.40)
Pf6N2E2_3762 +0.4
Aspartate transaminase:
L-aspartate + 2-oxoglutarate→oxaloacetate + L-glutamate (EC 2.6.1.1)
Pf6N2E2_1496 -0.1
Pf6N2E2_2127 +2.4
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 -0.3
Pf6N2E2_3173 +0.2
Pf6N2E2_4288 +0.1
Pf6N2E2_4386 -0.1
Pf6N2E2_462 +0.0
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 +0.0
Pf6N2E2_5890 -0.1
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 -0.0
Pf6N2E2_684 -0.0
Alanine transaminase (in reverse):
pyruvate + L-glutamate→L-alanine + 2-oxoglutarate (EC 2.6.1.2)
Pf6N2E2_2446 +0.0
Pf6N2E2_2862 -0.1
Phosphoenolpyruvate carboxykinase (ATP):
oxaloacetate + ATP→CO2 + phosphoenolpyruvate + ADP (EC 4.1.1.49)
Pf6N2E2_3899 +0.4
L-alanine[in]→L-alanine[out]
No genes
phosphoenolpyruvate[in]→phosphoenolpyruvate[out]
No genes
Alanine transaminase (in reverse):
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)
Pf6N2E2_2446 +0.0
Pyruvate, phosphate dikinase:
pyruvate + ATP + phosphate→phosphoenolpyruvate + AMP + diphosphate + H+ (EC 2.7.9.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information