MetaCyc Pathway: superpathway of aromatic amino acid biosynthesis in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cadmium chloride hemipentahydrate 0.000015625 M
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3-deoxy-7-phosphoheptulonate synthase:
D-erythrose 4-phosphate + phosphoenolpyruvate + H2O→3-deoxy-D-arabino-heptulosonate 7-phosphate + phosphate (EC 2.5.1.54)
Pf6N2E2_2025 -0.2
Pf6N2E2_2453 -0.2
Pf6N2E2_2470 +0.2
3-dehydroquinate synthase:
3-deoxy-D-arabino-heptulosonate 7-phosphate→3-dehydroquinate + phosphate (EC 4.2.3.4)
Pf6N2E2_1564 -0.2
Pf6N2E2_3754 N.D.
3-dehydroquinate dehydratase:
3-dehydroquinate→3-dehydroshikimate + H2O (EC 4.2.1.10)
Pf6N2E2_3048 N.D.
Pf6N2E2_3510 N.D.
Pf6N2E2_5062 -0.3
Shikimate dehydrogenase (in reverse):
3-dehydroshikimate + NADPH + H+→shikimate + NADP+ (EC 1.1.1.25)
Pf6N2E2_2233 +0.2
Pf6N2E2_4130 N.D.
Pf6N2E2_5061 -0.1
Shikimate kinase:
shikimate + ATP→shikimate 3-phosphate + ADP + H+ (EC 2.7.1.71)
Pf6N2E2_3755 N.D.
3-phosphoshikimate 1-carboxyvinyltransferase:
shikimate 3-phosphate + phosphoenolpyruvate→5-enolpyruvoyl-shikimate 3-phosphate + phosphate (EC 2.5.1.19)
Pf6N2E2_2518 -0.7
Chorismate synthase:
5-enolpyruvoyl-shikimate 3-phosphate→chorismate + phosphate (EC 4.2.3.5)
Pf6N2E2_5822 N.D.
Anthranilate synthase:
chorismate + L-glutamine→anthranilate + L-glutamate + H+ + pyruvate (EC 4.1.3.27)
Pf6N2E2_1717 -0.1
Pf6N2E2_1718 +0.2
Pf6N2E2_4822 -0.1
Pf6N2E2_4824 +0.2
Chorismate mutase:
chorismate→prephenate (EC 5.4.99.5)
Pf6N2E2_2520 N.D.
Pf6N2E2_3822 -0.0
Prephenate dehydratase:
prephenate + H+→3-phenyl-2-oxopropanoate + CO2 + H2O (EC 4.2.1.51)
Pf6N2E2_1300 -0.3
Pf6N2E2_2520 N.D.
Prephenate dehydrogenase:
prephenate + NAD+→3-(4-hydroxyphenyl)pyruvate + CO2 + NADH (EC 1.3.1.12)
Pf6N2E2_2518 -0.7
Anthranilate phosphoribosyltransferase (in reverse):
anthranilate + 5-phospho-α-D-ribose 1-diphosphate→N-(5-phosphoribosyl)-anthranilate + diphosphate (EC 2.4.2.18)
Pf6N2E2_304 -1.0
Pf6N2E2_4825 +0.0
Phosphoribosylanthranilate isomerase:
N-(5-phosphoribosyl)-anthranilate→1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate (EC 5.3.1.24)
Pf6N2E2_71 +0.1
Aspartate transaminase (in reverse):
3-phenyl-2-oxopropanoate + L-glutamate→L-phenylalanine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.57)
Pf6N2E2_1496 -0.1
Pf6N2E2_2127 +2.4
Pf6N2E2_2446 +0.0
Pf6N2E2_2519 +0.1
Pf6N2E2_2632 -0.3
Pf6N2E2_3173 +0.2
Pf6N2E2_3251 +0.7
Pf6N2E2_4288 +0.1
Pf6N2E2_4386 -0.1
Pf6N2E2_462 +0.0
Pf6N2E2_50 -0.2
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 +0.0
Pf6N2E2_5890 -0.1
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 -0.0
Pf6N2E2_684 -0.0
Aspartate transaminase (in reverse):
3-(4-hydroxyphenyl)pyruvate + L-glutamate→L-tyrosine + 2-oxoglutarate (EC 2.6.1.1; 2.6.1.27; 2.6.1.5; 2.6.1.57)
Pf6N2E2_1496 -0.1
Pf6N2E2_2127 +2.4
Pf6N2E2_2446 +0.0
Pf6N2E2_2519 +0.1
Pf6N2E2_2632 -0.3
Pf6N2E2_3173 +0.2
Pf6N2E2_3251 +0.7
Pf6N2E2_4288 +0.1
Pf6N2E2_4386 -0.1
Pf6N2E2_462 +0.0
Pf6N2E2_50 -0.2
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 +0.0
Pf6N2E2_5890 -0.1
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 -0.0
Pf6N2E2_684 -0.0
Indole-3-glycerol-phosphate synthase:
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H+→(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O (EC 4.1.1.48)
Pf6N2E2_4826 -0.1
Indole-3-glycerol-phosphate lyase:
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate→indole + D-glyceraldehyde 3-phosphate (EC 4.1.2.8; 4.2.1.20)
Pf6N2E2_3982 +0.3
Pf6N2E2_4047 -0.1
Pf6N2E2_4048 -0.1
Pf6N2E2_5238 +0.0
indole + L-serine→L-tryptophan + H2O (EC 4.2.1.122; 4.2.1.20)
Pf6N2E2_3982 +0.3
Pf6N2E2_4047 -0.1
Pf6N2E2_4048 -0.1
Pf6N2E2_5238 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information