MetaCyc Pathway: superpathway of L-methionine biosynthesis (transsulfuration) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cadmium chloride hemipentahydrate 0.000015625 M
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf6N2E2_1496 +0.1
Pf6N2E2_2127 +2.2
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 -0.5
Pf6N2E2_3173 +0.1
Pf6N2E2_4288 -0.1
Pf6N2E2_4386 -0.0
Pf6N2E2_462 +0.1
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 -0.0
Pf6N2E2_5890 -0.0
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 -0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 +0.2
Pf6N2E2_684 -0.0
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf6N2E2_5676 +0.4
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf6N2E2_2844 +0.2
Pf6N2E2_4232 -0.0
Pf6N2E2_67 N.D.
Pf6N2E2_68 +0.2
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf6N2E2_3143 N.D.
Pf6N2E2_4963 +0.3
Homoserine O-succinyltransferase:
L-homoserine + succinyl-CoA→O-succinyl-L-homoserine + coenzyme A (EC 2.3.1.46)
No genes
Cystathionine gamma-synthase:
O-succinyl-L-homoserine + L-cysteine→L-cystathionine + H+ + succinate (EC 2.5.1.48)
Pf6N2E2_4958 -0.0
Pf6N2E2_77 +0.2
Cysteine-S-conjugate beta-lyase:
L-cystathionine + H2O→L-homocysteine + ammonium + pyruvate (EC 4.4.1.13)
No genes
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf6N2E2_5472 +0.1
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
Pf6N2E2_2068 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information