MetaCyc Pathway: superpathway of L-methionine biosynthesis (by sulfhydrylation) in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes Cadmium chloride hemipentahydrate 0.000015625 M
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf6N2E2_1496 +0.1
Pf6N2E2_2127 +2.2
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 -0.5
Pf6N2E2_3173 +0.1
Pf6N2E2_4288 -0.1
Pf6N2E2_4386 -0.0
Pf6N2E2_462 +0.1
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 -0.0
Pf6N2E2_5890 -0.0
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 -0.1
Pf6N2E2_5989 +2.2
Pf6N2E2_637 +0.2
Pf6N2E2_684 -0.0
Sulfate adenylyltransferase:
sulfate + ATP + H+→adenosine 5'-phosphosulfate + diphosphate (EC 2.7.7.4)
Pf6N2E2_5257 -0.1
Pf6N2E2_5258 -0.2
Adenylyl-sulfate kinase:
adenosine 5'-phosphosulfate + ATP→3'-phosphoadenylyl-sulfate + ADP + H+ (EC 2.7.1.25)
Pf6N2E2_3654 +0.0
Pf6N2E2_5258 -0.2
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf6N2E2_5676 +0.4
Phosphoadenylyl-sulfate reductase (thioredoxin) (in reverse):
3'-phosphoadenylyl-sulfate + a reduced thioredoxin→sulfite + adenosine 3',5'-bisphosphate + an oxidized thioredoxin + 2 H+ (EC 1.8.4.8)
Pf6N2E2_6054 N.D.
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf6N2E2_2844 +0.2
Pf6N2E2_4232 -0.0
Pf6N2E2_67 N.D.
Pf6N2E2_68 +0.2
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf6N2E2_3143 N.D.
Pf6N2E2_4963 +0.3
Sulfite reductase (NADPH) (in reverse):
sulfite + 3 NADPH + 5 H+→hydrogen sulfide + 3 NADP+ + 3 H2O (EC 1.8.1.2)
Pf6N2E2_1511 +0.1
Pf6N2E2_2073 -0.5
Pf6N2E2_3335 +0.1
Homoserine O-acetyltransferase:
L-homoserine + acetyl-CoA→O-acetyl-L-homoserine + coenzyme A (EC 2.3.1.31)
Pf6N2E2_4596 -0.0
Pf6N2E2_4597 +0.1
O-acetylhomoserine aminocarboxypropyltransferase:
O-acetyl-L-homoserine + hydrogen sulfide→L-homocysteine + acetate + H+ (EC 2.5.1.49)
Pf6N2E2_1768 -0.2
Pf6N2E2_1876 -0.3
Pf6N2E2_77 +0.2
5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase:
L-homocysteine + 5-methyltetrahydropteroyl tri-L-glutamate→L-methionine + tetrahydropteroyl tri-L-glutamate (EC 2.1.1.14)
Pf6N2E2_5472 +0.1
Methionine synthase:
L-homocysteine + a 5-methyltetrahydrofolate→L-methionine + a tetrahydrofolate (EC 2.1.1.13)
Pf6N2E2_2068 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information