MetaCyc Pathway: glutathione-mediated detoxification I in Pseudomonas fluorescens FW300-N2E2

Reactions and Genes	Cadmium chloride hemipentahydrate 0.000015625 M
Glutathione transferase: RX + glutathione→a glutathione-S-conjugate + HX (EC 2.5.1.18)
Pf6N2E2_1445	-0.5
Pf6N2E2_1553	+0.2
Pf6N2E2_1561	+0.0
Pf6N2E2_1619	-0.0
Pf6N2E2_2148	+0.1
Pf6N2E2_2168	+0.1
Pf6N2E2_303	-0.0
Pf6N2E2_395	-0.1
Pf6N2E2_4178	+0.1
Pf6N2E2_4235	+0.0
Pf6N2E2_4428	-0.2
Pf6N2E2_509	+0.2
Pf6N2E2_5292	-0.6
Pf6N2E2_5412	-0.1
Pf6N2E2_5517	+0.1
Pf6N2E2_5974	-0.3
Pf6N2E2_6007	-0.2
Pf6N2E2_933	-0.0
a glutathione-S-conjugate[in] + ATP[in] + H2O[in]→a glutathione-S-conjugate[out] + ADP[in] + H+[in] + phosphate[in] (EC 7.6.2.3)
No genes
a glutathione-S-conjugate + H2O→an (L-cysteinylglycine)-S-conjugate + L-glutamate (EC 3.4.19.13)
No genes
an (L-cysteinylglycine)-S-conjugate + H2O→an L-cysteine-S-conjugate + glycine (EC 3.4.13.23)
No genes
Cysteine-S-conjugate N-acetyltransferase: an L-cysteine-S-conjugate + acetyl-CoA→a mercapturate + coenzyme A + H+ (EC 2.3.1.80)
No genes
Cysteine-S-conjugate beta-lyase: an L-cysteine-S-conjugate→2-aminoprop-2-enoate + a thiol (EC 4.4.1.13)
No genes
Tryptophanase: 2-aminoprop-2-enoate→2-iminopropanoate (spontaneous) (EC 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047	+0.3
Pf6N2E2_3918	+0.0
Pf6N2E2_4685	-0.1
Pf6N2E2_5557	-1.7
Pf6N2E2_5896	-0.0
2-iminopropanoate + H2O→pyruvate + ammonium (spontaneous) (EC 3.5.99.10; 4.1.99.1; 4.1.99.2; 4.3.1.13; 4.3.1.17; 4.3.1.18; 4.3.3.8; 4.4.1.1; 4.4.1.13; 4.4.1.15; 4.4.1.25; 4.4.1.28; 4.4.1.35; 4.5.1.2)
Pf6N2E2_2047	+0.3
Pf6N2E2_3918	+0.0
Pf6N2E2_4360	-1.0
Pf6N2E2_4685	-0.1
Pf6N2E2_5557	-1.7
Pf6N2E2_5896	-0.0

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information