MetaCyc Pathway: superpathway of L-isoleucine biosynthesis I in Pseudomonas fluorescens FW300-N2E2

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Reactions and Genes chromate 0.0000015625 M
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Aspartate transaminase (in reverse):
oxaloacetate + L-glutamate→L-aspartate + 2-oxoglutarate (EC 2.6.1.1)
Pf6N2E2_1496 -0.1
Pf6N2E2_2127 +3.1
Pf6N2E2_2446 +0.0
Pf6N2E2_2632 +0.0
Pf6N2E2_3173 +0.1
Pf6N2E2_4288 +0.0
Pf6N2E2_4386 -0.3
Pf6N2E2_462 +0.0
Pf6N2E2_5300 +1.6
Pf6N2E2_5813 -0.1
Pf6N2E2_5890 -0.2
Pf6N2E2_5891 N.D.
Pf6N2E2_5895 +0.0
Pf6N2E2_5989 +2.2
Pf6N2E2_637 +0.0
Pf6N2E2_684 +0.1
Aspartate kinase:
L-aspartate + ATP→L-aspartyl-4-phosphate + ADP (EC 2.7.2.4)
Pf6N2E2_5676 -0.1
Aspartate-semialdehyde dehydrogenase (in reverse):
L-aspartyl-4-phosphate + NADPH + H+→L-aspartate 4-semialdehyde + NADP+ + phosphate (EC 1.2.1.11)
Pf6N2E2_2844 +0.2
Pf6N2E2_4232 -0.1
Pf6N2E2_67 N.D.
Pf6N2E2_68 -0.1
Homoserine dehydrogenase (in reverse):
L-aspartate 4-semialdehyde + NAD(P)H + H+→L-homoserine + NAD(P)+ (EC 1.1.1.3)
Pf6N2E2_3143 N.D.
Pf6N2E2_4963 -0.4
Homoserine kinase:
L-homoserine + ATP→O-phospho-L-homoserine + ADP + H+ (EC 2.7.1.39)
Pf6N2E2_4164 N.D.
Pf6N2E2_6055 +0.3
Threonine synthase:
O-phospho-L-homoserine + H2O→L-threonine + phosphate (EC 4.2.3.1)
Pf6N2E2_3142 N.D.
Pf6N2E2_4961 +0.1
Pf6N2E2_5296 +0.2
Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
Pf6N2E2_1562 +0.1
Pf6N2E2_3938 -0.0
Pf6N2E2_802 +0.1
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Pf6N2E2_1562 +0.1
Pf6N2E2_1819 +0.6
Pf6N2E2_2208 +0.4
Pf6N2E2_3938 -0.0
Pf6N2E2_5896 -0.2
Pf6N2E2_802 +0.1
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
Pf6N2E2_1562 +0.1
Pf6N2E2_1819 +0.6
Pf6N2E2_2208 +0.4
Pf6N2E2_3938 -0.0
Pf6N2E2_4360 -1.2
Pf6N2E2_5896 -0.2
Pf6N2E2_802 +0.1
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
Pf6N2E2_5175 +2.6
Pf6N2E2_5176 +2.1
Pf6N2E2_5475 -0.2
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
Pf6N2E2_5177 +1.1
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
Pf6N2E2_1668 +0.1
Pf6N2E2_4564 +3.0
Pf6N2E2_609 -0.3
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
Pf6N2E2_477 -0.2

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information