MetaCyc Pathway: toluene degradation III (aerobic) (via p-cresol) in Sinorhizobium meliloti 1021

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Reactions and Genes Protocatechuic Acid (C)
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toluene + NADH + dioxygen + H+→4-methylphenol + NAD+ + H2O (EC 1.14.13.236)
No genes
4-methylphenol + 2 an oxidized azurin + H2O→4-hydroxybenzyl alcohol + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzyl alcohol + 2 an oxidized azurin→4-hydroxybenzaldehyde + 2 H+ + 2 a reduced azurin (EC 1.17.9.1)
No genes
4-hydroxybenzaldehyde + NADP+ + H2O→4-hydroxybenzoate + NADPH + 2 H+ (EC 1.2.1.96)
No genes
4-hydroxybenzoate 3-monooxygenase:
4-hydroxybenzoate + NADPH + dioxygen + H+→protocatechuate + NADP+ + H2O (EC 1.14.13.2)
SM_b20583 -0.1
Protocatechuate 3,4-dioxygenase:
protocatechuate + dioxygen→3-carboxy-cis,cis-muconate + 2 H+ (EC 1.13.11.3)
SM_b20576 -1.6
SM_b20577 -2.7
SM_b21380 +0.2
3-carboxy-cis,cis-muconate cycloisomerase (in reverse):
3-carboxy-cis,cis-muconate + H+→(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate (EC 5.5.1.2)
SM_b20575 N.D.
4-carboxymuconolactone decarboxylase:
(2-carboxy-2,5-dihydro-5-oxofuran-2-yl)-acetate + H+→(4,5-dihydro-5-oxofuran-2-yl)-acetate + CO2 (EC 4.1.1.44)
SM_b20578 -1.6
SMa0316 +0.2
SMa0559 +0.1
3-oxoadipate enol-lactonase:
(4,5-dihydro-5-oxofuran-2-yl)-acetate + H2O→3-oxoadipate + H+ (EC 3.1.1.24)
SM_b20579 -3.1
SMa0792 +0.1
SMa1327 -0.1
3-oxoadipate CoA-transferase:
3-oxoadipate + succinyl-CoA→3-oxoadipoyl-CoA + succinate (EC 2.8.3.6)
SM_b20587 -5.6
SM_b20588 -4.7
3-oxoadipyl-CoA thiolase (in reverse):
3-oxoadipoyl-CoA + coenzyme A→acetyl-CoA + succinyl-CoA (EC 2.3.1.174)
SM_b20589 -5.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information