MetaCyc Pathway: biotin biosynthesis II in Ralstonia sp. UNC404CL21Col

Reactions and Genes	Octanoic (C)
6-carboxyhexanoate--CoA ligase: pimelate + ATP + coenzyme A→pimeloyl-CoA + AMP + diphosphate (EC 6.2.1.14)
No genes
8-amino-7-oxononanoate synthase: pimeloyl-CoA + L-alanine + H+→(8S)-8-amino-7-oxononanoate + CO2 + coenzyme A (EC 2.3.1.47)
ABZR87_RS12340	-2.8
(8S)-8-amino-7-oxononanoate + L-lysine→(7R,8S)-diaminononanoate + (S)-2-amino-6-oxohexanoate (EC 2.6.1.105)
ABZR87_RS04915	+0.1
Dethiobiotin synthase: (7R,8S)-diaminononanoate + ATP + CO2→dethiobiotin + ADP + 3 H+ + phosphate (EC 6.3.3.3)
ABZR87_RS12345	-4.1
Biotin synthase: dethiobiotin + H+ + 2 a reduced [2Fe-2S] ferredoxin + S-adenosyl-L-methionine + a sulfurated [sulfur carrier]→4,5-secobiotin + 5'-deoxyadenosine + L-methionine + 2 an oxidized [2Fe-2S] ferredoxin + an unsulfurated [sulfur carrier] (EC 2.8.1.6)
ABZR87_RS06265	-3.5
Biotin synthase: 4,5-secobiotin + S-adenosyl-L-methionine→biotin + 5'-deoxyadenosine + L-methionine + H+ (EC 2.8.1.6)
ABZR87_RS06265	-3.5

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information