MetaCyc Pathway: L-isoleucine biosynthesis I (from threonine) in Sinorhizobium meliloti 1021

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Reactions and Genes L-Arabinose (C)
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Threonine ammonia-lyase:
L-threonine→(Z)-2-aminobutenoate + H2O (EC 4.3.1.19)
SM_b20432 -0.0
SMa1872 +0.0
SMc00936 +0.1
1-aminocyclopropane-1-carboxylate deaminase:
(Z)-2-aminobutenoate→2-iminobutanoate (spontaneous) (EC 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
SM_b20432 -0.0
SMa1872 +0.0
SMc00936 +0.1
SMc01666 +0.0
2-iminobutanoate + H2O→2-oxobutanoate + ammonium (spontaneous) (EC 3.5.99.10; 3.5.99.7; 4.3.1.19; 4.4.1.1; 4.4.1.11; 4.4.1.2)
SM_b20432 -0.0
SMa1872 +0.0
SMc00936 +0.1
SMc01666 +0.0
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
SM_b20400 +0.0
SMa0958 -0.2
SMa2211 +0.3
SMc01430 -2.6
SMc01431 -6.3
SMc02263 +0.3
SMc04455 +0.1
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
SMc04346 -2.9
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
SM_b20115 +0.1
SM_b20890 -4.6
SMa0235 -0.2
SMc00884 -0.3
SMc04045 -6.4
SMc04144 +0.2
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
SMc00042 +0.1
SMc02896 +0.0

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information