MetaCyc Pathway: lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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D-lactate dehydrogenase (cytochrome c-553):
(R)-lactate + 2 an oxidized cytochrome c-553→pyruvate + 2 H+ + 2 a reduced cytochrome c-553 (EC 1.1.2.5)
BWI76_RS12790 +0.1
BWI76_RS19515 -0.1
L-lactate dehydrogenase (cytochrome):
(S)-lactate + 2 an oxidized c-type cytochrome→pyruvate + 2 a reduced c-type cytochrome + 2 H+ (EC 1.1.2.3)
BWI76_RS15220 +0.5
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + 2 a reduced ferredoxin [iron-sulfur] cluster + H+ (EC 1.2.7.1)
BWI76_RS12730 +0.0
BWI76_RS18805 -1.0
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 +0.1
BWI76_RS20240 N.D.
BWI76_RS20805 -0.1
BWI76_RS23150 +0.2
CO2 + 2 a reduced type I cytochrome c3 + H+→formate + 2 an oxidized type I cytochrome c3 (EC 1.17.2.3)
No genes
a reduced ferredoxin [iron-sulfur] cluster + an oxidized type I cytochrome c3→a reduced type I cytochrome c3 + an oxidized ferredoxin [iron-sulfur] cluster
No genes
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 -0.3
BWI76_RS20235 N.D.
BWI76_RS20815 +0.1
BWI76_RS20820 +0.1
BWI76_RS23200 +0.2
Cytochrome-c3 hydrogenase (in reverse):
2 a reduced type I cytochrome c3 + 2 H+→H2 + 2 an oxidized type I cytochrome c3 (EC 1.12.2.1)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information