MetaCyc Pathway: glycerol degradation to butanol in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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Glycerol dehydrogenase:
glycerol + NAD+→dihydroxyacetone + NADH + H+ (EC 1.1.1.6)
BWI76_RS00815 +0.2
BWI76_RS07795 +0.2
BWI76_RS24585 +0.0
Glycerone kinase:
dihydroxyacetone + ATP→glycerone phosphate + ADP + H+ (EC 2.7.1.29)
BWI76_RS24610 -0.1
Triose-phosphate isomerase (in reverse):
glycerone phosphate→D-glyceraldehyde 3-phosphate (EC 5.3.1.1)
BWI76_RS03100 +0.3
BWI76_RS27465 N.D.
Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):
D-glyceraldehyde 3-phosphate + NAD+ + phosphate→3-phospho-D-glyceroyl phosphate + NADH + H+ (EC 1.2.1.12)
BWI76_RS11505 N.D.
BWI76_RS12975 -0.2
BWI76_RS13215 +0.1
Phosphoglycerate kinase (in reverse):
3-phospho-D-glyceroyl phosphate + ADP→3-phospho-D-glycerate + ATP (EC 2.7.2.3)
BWI76_RS23980 N.D.
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
BWI76_RS04200 +0.2
BWI76_RS08605 +1.1
BWI76_RS13025 +0.1
BWI76_RS27230 -0.0
Phosphopyruvate hydratase:
2-phospho-D-glycerate→phosphoenolpyruvate + H2O (EC 4.2.1.11)
BWI76_RS22790 N.D.
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP (EC 2.7.1.40)
BWI76_RS16960 N.D.
BWI76_RS18115 -0.0
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 +0.0
BWI76_RS18805 -1.0
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 -0.3
BWI76_RS13135 +0.3
BWI76_RS16135 +0.5
BWI76_RS20460 +0.1
BWI76_RS23445 +0.1
BWI76_RS23710 -0.3
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information