MetaCyc Pathway: naphthalene degradation to acetyl-CoA in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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Naphthalene 1,2-dioxygenase:
naphthalene + NADH + dioxygen + H+→(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+
(EC 1.14.12.12)
No genes
Cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase:
(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD+→naphthalene-1,2-diol + NADH + H+
(EC 1.3.1.29)
No genes
naphthalene-1,2-diol + dioxygen→2-hydroxychromene-2-carboxylate + H+ (EC 1.13.11.56)
No genes
2-hydroxychromene-2-carboxylate→trans-O-hydroxybenzylidenepyruvate (EC 5.99.1.4)
No genes
trans-O-hydroxybenzylidenepyruvate + H2O→salicylaldehyde + pyruvate (EC 4.1.2.45)
No genes
Salicylaldehyde dehydrogenase:
salicylaldehyde + NAD+ + H2O→salicylate + NADH + 2 H+
(EC 1.2.1.65)
No genes
Salicylate 1-monooxygenase:
salicylate + NADH + dioxygen + 2 H+→catechol + CO2 + NAD+ + H2O
(EC 1.14.13.1)
BWI76_RS10500 -0.2
Catechol 2,3-dioxygenase:
catechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
(EC 1.13.11.2)
BWI76_RS12910 +0.2
2-hydroxymuconate-semialdehyde hydrolase:
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + formate + H+
(EC 3.7.1.9)
No genes
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate
(EC 4.2.1.80)
BWI76_RS16910 -0.4
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate
(EC 4.1.3.39)
BWI76_RS16900 -0.5
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+
(EC 1.2.1.10)
BWI76_RS02835 +0.2
BWI76_RS03590 +0.1
BWI76_RS16905 +0.1
BWI76_RS17250 -1.5
BWI76_RS20790 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information