MetaCyc Pathway: pyruvate fermentation to hexanol (engineered) in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 +0.0
BWI76_RS18805 -1.0
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 -0.3
BWI76_RS13135 +0.3
BWI76_RS16135 +0.5
BWI76_RS20460 +0.1
BWI76_RS23445 +0.1
BWI76_RS23710 -0.3
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
crotonyl-CoA + NADH + H+→butanoyl-CoA + NAD+ (EC 1.3.1.44)
BWI76_RS05470 +0.0
BWI76_RS16860 -0.1
Acetyl-CoA C-acyltransferase:
acetyl-CoA + butanoyl-CoA→3-oxohexanoyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 -0.3
BWI76_RS13135 +0.3
BWI76_RS16135 +0.5
BWI76_RS20460 +0.1
BWI76_RS23445 +0.1
BWI76_RS23710 -0.3
3-hydroxyacyl-CoA dehydrogenase (in reverse):
3-oxohexanoyl-CoA + NADH + H+→(S)-3-hydroxyhexanoyl-CoA + NAD+ (EC 1.1.1.35)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
Long-chain-enoyl-CoA hydratase:
(S)-3-hydroxyhexanoyl-CoA→(2E)-hex-2-enoyl-CoA + H2O (EC 4.2.1.74)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
Trans-2-enoyl-CoA reductase (NAD(+)) (in reverse):
(2E)-hex-2-enoyl-CoA + NADH + H+→hexanoyl-CoA + NAD+ (EC 1.3.1.44)
No genes
hexanoyl-CoA + NAD(P)H + H+→1-hexanal + coenzyme A + NAD(P)+
No genes
1-hexanal + NADH + H+→hexan-1-ol + NAD+
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information