MetaCyc Pathway: superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 +0.0
BWI76_RS18805 -1.0
Acetaldehyde dehydrogenase (acetylating) (in reverse):
acetyl-CoA + NADH + H+→acetaldehyde + coenzyme A + NAD+ (EC 1.2.1.10)
BWI76_RS02835 +0.2
BWI76_RS03590 +0.1
BWI76_RS16905 +0.1
BWI76_RS17250 -1.5
BWI76_RS20790 +0.4
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A (EC 2.3.1.16; 2.3.1.9)
BWI76_RS01360 -0.3
BWI76_RS13135 +0.3
BWI76_RS16135 +0.5
BWI76_RS20460 +0.1
BWI76_RS23445 +0.1
BWI76_RS23710 -0.3
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 +0.1
BWI76_RS20240 N.D.
BWI76_RS20805 -0.1
BWI76_RS23150 +0.2
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 -0.3
BWI76_RS20235 N.D.
BWI76_RS20815 +0.1
BWI76_RS20820 +0.1
BWI76_RS23200 +0.2
Alcohol dehydrogenase (in reverse):
acetaldehyde + NADH + H+→ethanol + NAD+ (EC 1.1.1.1)
BWI76_RS00645 -0.1
BWI76_RS02835 +0.2
BWI76_RS03140 -0.1
BWI76_RS03320 -0.1
BWI76_RS12395 +0.1
BWI76_RS13800 -0.1
BWI76_RS15240 +0.2
BWI76_RS17250 -1.5
BWI76_RS25510 +0.4
BWI76_RS27960 +0.2
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+ (EC 1.1.1.35)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
Butyrate--acetoacetate CoA-transferase (in reverse):
acetate + acetoacetyl-CoA→acetyl-CoA + acetoacetate (EC 2.8.3.9)
BWI76_RS23700 -0.1
(S)-3-hydroxybutanoyl-CoA→crotonyl-CoA + H2O (EC 4.2.1.150)
BWI76_RS01365 -0.1
BWI76_RS01370 N.D.
BWI76_RS20455 +0.2
crotonyl-CoA[cytosol] + 2 NADH[cytosol] + 2 an oxidized ferredoxin [iron-sulfur] cluster[cytosol]→butanoyl-CoA[cytosol] + 2 NAD+[cytosol] + 2 a reduced ferredoxin [iron-sulfur] cluster[cytosol] (EC 1.3.1.109)
No genes
Butanal dehydrogenase (in reverse):
butanoyl-CoA + NAD(P)H + H+→1-butanal + coenzyme A + NAD(P)+ (EC 1.2.1.57)
No genes
Phosphate butyryltransferase:
butanoyl-CoA + phosphate→butanoyl phosphate + coenzyme A (EC 2.3.1.19)
No genes
Branched-chain-fatty-acid kinase (in reverse):
butanoyl phosphate + ADP→butanoate + ATP (EC 2.7.2.14; 2.7.2.7)
No genes
Alcohol dehydrogenase (NADP(+)) (in reverse):
1-butanal + NADPH + H+→butan-1-ol + NADP+ (EC 1.1.1.2)
No genes
1-butanal + NADH + H+→butan-1-ol + NAD+
No genes
Butyrate--acetoacetate CoA-transferase (in reverse):
acetoacetyl-CoA + butanoate→acetoacetate + butanoyl-CoA (EC 2.8.3.9)
No genes
Acetoacetate decarboxylase:
acetoacetate + H+→acetone + CO2 (EC 4.1.1.4)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information