MetaCyc Pathway: catechol degradation II (meta-cleavage pathway) in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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Catechol 2,3-dioxygenase:
catechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+
(EC 1.13.11.2)
BWI76_RS12910 +0.2
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + NAD+ + H2O→(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+ (EC 1.2.1.85)
No genes
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate→(3E)-2-oxohex-3-enedioate (EC 5.3.2.6)
No genes
4-oxalocrotonate decarboxylase:
(3E)-2-oxohex-3-enedioate + H+→(2Z)-2-hydroxypenta-2,4-dienoate + CO2
(EC 4.1.1.77)
No genes
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate
(EC 4.2.1.80)
BWI76_RS16910 -0.4
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate
(EC 4.1.3.39)
BWI76_RS16900 -0.5
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+
(EC 1.2.1.10)
BWI76_RS02835 +0.2
BWI76_RS03590 +0.1
BWI76_RS16905 +0.1
BWI76_RS17250 -1.5
BWI76_RS20790 +0.4

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information