MetaCyc Pathway: reductive TCA cycle II in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with 0.1 mM glass beads
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2-oxoglutarate synthase (in reverse):
succinyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→2-oxoglutarate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.3)
No genes
2-oxoglutarate carboxylase:
2-oxoglutarate + ATP + CO2 + H2O→oxalosuccinate + ADP + 2 H+ + phosphate (EC 6.4.1.7)
No genes
oxalosuccinate + NADH + H+→D-threo-isocitrate + NAD+
No genes
Aconitate hydratase (in reverse):
D-threo-isocitrate→cis-aconitate + H2O (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.2
Aconitate hydratase (in reverse):
cis-aconitate + H2O→citrate (EC 4.2.1.3)
BWI76_RS04910 N.D.
BWI76_RS11940 +0.2
Citrate--CoA ligase:
citrate + ATP + coenzyme A→(3S)-citryl-CoA + ADP + phosphate (EC 6.2.1.18)
No genes
Citryl-CoA lyase:
(3S)-citryl-CoA→acetyl-CoA + oxaloacetate (EC 4.1.3.34)
No genes
Malate dehydrogenase (in reverse):
oxaloacetate + NADH + H+→(S)-malate + NAD+ (EC 1.1.1.37; 1.1.1.38)
BWI76_RS13830 +0.1
BWI76_RS16775 +0.3
BWI76_RS25420 +0.2
Pyruvate synthase (in reverse):
acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 +0.0
BWI76_RS18805 -1.0
Fumarate hydratase:
(S)-malate→fumarate + H2O (EC 4.2.1.2)
BWI76_RS08450 +0.3
BWI76_RS16115 +0.2
BWI76_RS16270 +0.1
BWI76_RS16275 +0.3
BWI76_RS16955 +0.0
Succinate dehydrogenase (ubiquinone):
fumarate[in] + a menaquinol→succinate[in] + a menaquinone (EC 1.3.5.1)
BWI76_RS02440 -0.5
BWI76_RS02445 -0.7
BWI76_RS02450 -0.4
BWI76_RS02455 -0.1
Succinate--CoA ligase (ADP-forming):
succinate + ATP + coenzyme A→succinyl-CoA + ADP + phosphate (EC 6.2.1.5)
BWI76_RS08425 -0.1
BWI76_RS08430 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information