MetaCyc Pathway: 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand
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3-phenylpropanoate dioxygenase:
3-phenylpropanoate + NADH + dioxygen + H+→3-(5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+
(EC 1.14.12.19)
No genes
3-(3-hydroxyphenyl)propanoate + NADH + dioxygen + H+→3-(2,3-dihydroxyphenyl)propanoate + NAD+ + H2O (EC 1.14.13.127)
BWI76_RS16925 +0.1
3-(5,6-dihydroxycyclohexa-1,3-dien-1-yl)propanoate + NAD+→3-(2,3-dihydroxyphenyl)propanoate + NADH + H+ (EC 1.3.1.87)
No genes
3-carboxyethylcatechol 2,3-dioxygenase:
3-(2,3-dihydroxyphenyl)propanoate + dioxygen→(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H+
(EC 1.13.11.16)
BWI76_RS16920 +0.2
(2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + H+ + succinate (EC 3.7.1.14)
BWI76_RS16915 -0.6
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate
(EC 4.2.1.80)
BWI76_RS16910 +0.0
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate
(EC 4.1.3.39)
BWI76_RS16900 -0.3
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+
(EC 1.2.1.10)
BWI76_RS02835 +0.1
BWI76_RS03590 +0.1
BWI76_RS16905 +0.0
BWI76_RS17250 -2.3
BWI76_RS20790 -0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information