MetaCyc Pathway: 3-methylbutanol biosynthesis (engineered) in Klebsiella michiganensis M5al

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Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand
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2-isopropylmalate synthase:
3-methyl-2-oxobutanoate + acetyl-CoA + H2O→(2S)-2-isopropylmalate + coenzyme A + H+ (EC 2.3.3.13)
BWI76_RS04680 -0.5
BWI76_RS22105 +0.1
3-isopropylmalate dehydratase (in reverse):
(2S)-2-isopropylmalate→2-isopropylmaleate + H2O (EC 4.2.1.33)
BWI76_RS04665 -0.7
BWI76_RS04670 -0.8
3-isopropylmalate dehydratase (in reverse):
2-isopropylmaleate + H2O→(2R,3S)-3-isopropylmalate (EC 4.2.1.33)
BWI76_RS04665 -0.7
BWI76_RS04670 -0.8
3-isopropylmalate dehydrogenase:
(2R,3S)-3-isopropylmalate + NAD+→(2S)-2-isopropyl-3-oxosuccinate + NADH + H+ (EC 1.1.1.85)
BWI76_RS04675 -0.8
3-isopropylmalate dehydrogenase:
(2S)-2-isopropyl-3-oxosuccinate + H+→4-methyl-2-oxopentanoate + CO2 (spontaneous) (EC 1.1.1.85)
BWI76_RS04675 -0.8
Branched-chain-2-oxoacid decarboxylase:
4-methyl-2-oxopentanoate + H+→3-methylbutanal + CO2 (EC 4.1.1.72)
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Alcohol dehydrogenase (in reverse):
3-methylbutanal + NADH + H+→3-methylbutanol + NAD+ (EC 1.1.1.1)
BWI76_RS00645 +0.2
BWI76_RS02835 +0.1
BWI76_RS03140 -0.0
BWI76_RS03320 +0.0
BWI76_RS12395 +0.0
BWI76_RS13800 -0.2
BWI76_RS15240 +0.1
BWI76_RS17250 -2.3
BWI76_RS25510 +0.2
BWI76_RS27960 +0.1

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information