| Reactions and Genes | RCH2_defined_Glucose_noNitrogen in Eppendorf tube with sand |
|---|
| remove |
| toluene + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→benzyl alcohol + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.14.15.26) |
| No genes |
Aryl-alcohol dehydrogenase:
benzyl alcohol + NAD+→benzaldehyde + NADH + H+ (EC 1.1.1.90) |
| BWI76_RS02040 | -0.1 |
Benzaldehyde dehydrogenase (NAD(+)):
benzaldehyde + NAD+ + H2O→benzoate + NADH + 2 H+ (EC 1.2.1.28) |
| No genes |
Benzoate 1,2-dioxygenase:
benzoate + NADH + dioxygen + H+→3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+ (EC 1.14.12.10) |
| BWI76_RS13705 | -0.1 |
| BWI76_RS13710 | -0.0 |
| BWI76_RS13715 | -0.3 |
1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate dehydrogenase:
3,5-cyclohexadiene-1,2-diol-1-carboxylate + NAD+→catechol + CO2 + NADH (EC 1.3.1.25) |
| BWI76_RS13720 | -0.5 |
Catechol 2,3-dioxygenase:
catechol + dioxygen→(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H+ (EC 1.13.11.2) |
| BWI76_RS12910 | -0.1 |
2-hydroxymuconate-semialdehyde hydrolase:
(2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + H2O→(2Z)-2-hydroxypenta-2,4-dienoate + formate + H+ (EC 3.7.1.9) |
| No genes |
| (2Z,4E)-2-hydroxy-6-oxohexa-2,4-dienoate + NAD+ + H2O→(2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+ (EC 1.2.1.85) |
| No genes |
| (2Z,4E)-2-hydroxyhexa-2,4-dienedioate→(3E)-2-oxohex-3-enedioate (EC 5.3.2.6) |
| No genes |
4-oxalocrotonate decarboxylase:
(3E)-2-oxohex-3-enedioate + H+→(2Z)-2-hydroxypenta-2,4-dienoate + CO2 (EC 4.1.1.77) |
| No genes |
2-oxopent-4-enoate hydratase (in reverse):
(2Z)-2-hydroxypenta-2,4-dienoate + H2O→(S)-4-hydroxy-2-oxopentanoate (EC 4.2.1.80) |
| BWI76_RS16910 | +0.0 |
4-hydroxy-2-oxovalerate aldolase:
(S)-4-hydroxy-2-oxopentanoate→acetaldehyde + pyruvate (EC 4.1.3.39) |
| BWI76_RS16900 | -0.3 |
Acetaldehyde dehydrogenase (acetylating):
acetaldehyde + coenzyme A + NAD+→acetyl-CoA + NADH + H+ (EC 1.2.1.10) |
| BWI76_RS02835 | +0.1 |
| BWI76_RS03590 | +0.1 |
| BWI76_RS16905 | +0.0 |
| BWI76_RS17250 | -2.3 |
| BWI76_RS20790 | -0.1 |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information