MetaCyc Pathway: superpathway of L-alanine fermentation (Stickland reaction) in Klebsiella michiganensis M5al

Add experiment(s):


Reactions and Genes RCH2_defined_Glucose_noNitrogen in Eppendorf tube
remove
Alanine transaminase:
L-alanine + 2-oxoglutarate→pyruvate + L-glutamate (EC 2.6.1.2)
BWI76_RS20205 -0.1
BWI76_RS20495 -0.2
D-lactate dehydrogenase (in reverse):
pyruvate + NADH + H+→(R)-lactate + NAD+ (EC 1.1.1.28)
BWI76_RS12790 +0.4
BWI76_RS19515 +0.1
Pyruvate synthase:
pyruvate + coenzyme A + 2 an oxidized ferredoxin [iron-sulfur] cluster→acetyl-CoA + CO2 + H+ + 2 a reduced ferredoxin [iron-sulfur] cluster (EC 1.2.7.1)
BWI76_RS12730 -0.0
BWI76_RS18805 -1.2
Propionate CoA-transferase:
(R)-lactate + propanoyl-CoA→(R)-lactoyl-CoA + propanoate (EC 2.8.3.1)
BWI76_RS23700 -0.2
Phosphate acetyltransferase:
acetyl-CoA + phosphate→acetyl phosphate + coenzyme A (EC 2.3.1.8)
BWI76_RS03560 -0.1
BWI76_RS20240 N.D.
BWI76_RS20805 +0.5
BWI76_RS23150 -0.0
Acetate kinase (in reverse):
acetyl phosphate + ADP→acetate + ATP (EC 2.7.2.1; 2.7.2.15)
BWI76_RS18090 -0.0
BWI76_RS20235 N.D.
BWI76_RS20815 -0.0
BWI76_RS20820 +0.0
BWI76_RS23200 +0.0
Lactoyl-CoA dehydratase:
(R)-lactoyl-CoA→acryloyl-CoA + H2O (EC 4.2.1.54)
No genes
acryloyl-CoA + NADH + H+→propanoyl-CoA + NAD+ (EC 1.3.1.95)
No genes
Propionate CoA-transferase (in reverse):
propanoyl-CoA + acetate→propanoate + acetyl-CoA (EC 2.8.3.1)
BWI76_RS23700 -0.2

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information